Physiological responses of Celtis caucasica L. and Robinia pseudoacacia L. to the cadmium and lead stresses

Abstract

Afforestation of contaminated areas is considered as a possible strategy for reduction of contaminations. In the present study, the effects of lead (Pb) and cadmium (Cd) were investigated on chlorophyll fluorescence parameters (Fv/Fm, Fo, and Fm), photosynthetic pigments (chlorophyll a, b, and total chlorophyll), and proline in one-year-old seedlings of Celtis caucasica and Robinia pseudoacacia. The seedlings were treated 2 times during 10 days, with different concentrations of Pb and Cd (0, 250, 500, 1000 and 2000 mg L-1). Saline solutions containing Pb and Cd were sprayed on the leaves. Chlorophyll fluorescence was measured every other day. Chlorophyll and proline were also measured at the end of experiment period. The results indicated that chlorophyll fluorescence of C. caucasica and R. pseaudoacacia was affected by Pb on the last days and by Cd on the first days. The chlorophyll a content of C. caucasica at 250 mg L-1 of Pb and the chlorophyll a of R. pseudoacacia at 1000 and 2000 mg L-1 of Cd increased. With increasing Cd and Pb concentrations, proline of C. caucasica increased significantly while proline of R. pseudoacacia was not affected by Cd and Pb. Our results suggested fairly similar photosynthetic responses of C. caucasica and R. pseudoacacia to Cd and Pb concentrations. We concluded that physiological sensitivity of the both species to Pb and Cd were weak and can be used for afforestation in semi-arid areas contaminated by Pb and Cd.

Keywords

• Proline
• Chlorophyll
• Heavy metals
• Robinia pseudoacacia
• Celtis caucasica