The in vivo measurement of replication fork velocity and pausing by lag-time analysis

Dean Huang; Anna E. Johnson; Brandon S. Sim; Teresa W. Lo; Houra Merrikh; Paul A. Wiggins

doi:10.1038/s41467-023-37456-2

Nature Communications (Mar 2023)

The in vivo measurement of replication fork velocity and pausing by lag-time analysis

Dean Huang,
Anna E. Johnson,
Brandon S. Sim,
Teresa W. Lo,
Houra Merrikh,
Paul A. Wiggins

Affiliations

Dean Huang: Department of Physics, University of Washington
Anna E. Johnson: Department of Biochemistry, Vanderbilt University
Brandon S. Sim: Department of Physics, University of Washington
Teresa W. Lo: Department of Physics, University of Washington
Houra Merrikh: Department of Biochemistry, Vanderbilt University
Paul A. Wiggins: Department of Physics, University of Washington

DOI: https://doi.org/10.1038/s41467-023-37456-2
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 13

Abstract

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Lag-time analysis was developed to measure in vivo replisome dynamics. Observed dynamics are both locus and cell-cycle dependent: Pauses of seconds are observed at wild-type ribosomal DNA loci, as well as temporal fork velocity oscillations.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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