PLoS ONE (Jan 2011)

DNA extraction columns contaminated with murine sequences.

  • Otto Erlwein,
  • Mark J Robinson,
  • Simon Dustan,
  • Jonathan Weber,
  • Steve Kaye,
  • Myra O McClure

DOI
https://doi.org/10.1371/journal.pone.0023484
Journal volume & issue
Vol. 6, no. 8
p. e23484

Abstract

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Sequences of the novel gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV) have been described in human prostate cancer tissue, although the amounts of DNA are low. Furthermore, XMRV sequences and polytropic (p) murine leukemia viruses (MLVs) have been reported in patients with chronic fatigue syndrome (CFS). In assessing the prevalence of XMRV in prostate cancer tissue samples we discovered that eluates from naïve DNA purification columns, when subjected to PCR with primers designed to detect genomic mouse DNA contamination, occasionally gave rise to amplification products. Further PCR analysis, using primers to detect XMRV, revealed sequences derived from XMRV and pMLVs from mouse and human DNA and DNA of unspecified origin. Thus, DNA purification columns can present problems when used to detect minute amounts of DNA targets by highly sensitive amplification techniques.