Identification of Stable Quantitative Trait Loci for Sheath Blight Resistance Using Recombinant Inbred Line

Abstract

To identify stable quantitative trait loci (QTLs) responsible for sheath blight resistance, a recombinant inbred line mapping population consisting of 219 lines was developed by crossing Lemont and Yangdao 4. Average disease rating, average lesion length, maximum disease rating and maximum lesion length were assayed in six different environments. A total of 128 minor effect QTLs were detected by multiple interval mapping. These QTLs explained less than 11.2% of the phenotypic variations individually, and 106 QTLs were clustered in 20 QTL-rich regions/putative loci. Significant QTL × environment interactions were detected at three putative loci (qSBR11.1, qSBR11.2 and qSBR11.3), indicating that these three loci were not stable. The other 17 stable loci (qSBR1.1, qSBR1.2, qSBR2.1, qSBR2.3, qSBR3.1, qSBR3.2, qSBR3.5, qSBR3.6, qSBR5.1, qSBR7.1, qSBR8.1, qSBR9.1, qSBR9.2, qSBR9.3, qSBR12.1, qSBR12.2 and qSBR12.4) provided a foundation for marker-assisted selection in breeding. Analysis of allelic effect on the 20 putative loci identified 7 highly stable loci, including qSBR3.2, qSBR7.1, qSBR8.1, qSBR9.2, qSBR9.3, qSBR12.1 and qSBR12.2. Keywords: Rice, Sheath blight resistance, Quantitative trait locus, Recombinant inbred line