Analysis of 9 microsatellite markers in Kalmyk cattle

Abstract

A genetic analysis of the Kalmyk cattle population for 9 microsatellite loci: BM1824, BM 2113, INRA023, SPS 115, TGLA 122, TGLA 126, TGLA 227ETH 10, ETH 225 was carried out on the basis of the youth scientific laboratory of molecular genetics of the Kalmyk State University. 60 animals of the Kalmyk breed of breeding reproducer “Plodovitoe” of the Republic of Kalmykia were subjected to genetic testing. The loci INRA 023, TGLA 122, and TGLA 227 turned out to be the most informative for the Kalmyk breed. A comparison of the observed and expected heterozygosity in the Kalmyk breed shows that all 9 microsatellite loci showed an excess of the expected heterozygosity value over the observed one. The number of possible genotypes (Ng) has the highest value at the TGLA 122 locus, the indicator is 171, a smaller number of possible genotypes is observed at the TGLA 126 locus. Thus, microsatellite analysis showed a high level of genetic diversity in the studied herd of Kalmyk cattle. The data obtained indicate the possibility of using microsatellite DNA analysis for genetic examination of breeding animals by origin and analysis of pedigrees in order to study the transmission of traits by inheritance.