First record of <i>Helicotylenchus varicaudatus</i> Yuen, 1964 (Nematoda: Hoplolaimidae) parasitizing <i>Ammophila arenaria<i> (L.) Link in Portuguese coastal sand dunes

Abstract

A spiral nematode, Helicotylenchus varicaudatus Yuen, 1964, parasitizing Ammophila arenaria (L.) Link, the dominant grass in the Portuguese coastal sand dunes, is reported from Portugal for the first time and raises to seven the number of Helicotylenchus species detected in Portugal. A redescription of the species, with illustrations, and light and scanning electron microscope images of both female and male specimens, is presented. The rDNA containing the internal transcribed spacer regions (ITS) of H. varicaudatus was analysed with ITS-RFLP using the restriction endonuclease Hinf I. Molecular data from the ribosomal small subunit (SSU) (18S) confirmed the identification.

Keywords

• marram grass
• ITS-RFLP
• morphobiometry
• SSU (18S)