Nature Communications (Apr 2019)

CUT&Tag for efficient epigenomic profiling of small samples and single cells

  • Hatice S. Kaya-Okur,
  • Steven J. Wu,
  • Christine A. Codomo,
  • Erica S. Pledger,
  • Terri D. Bryson,
  • Jorja G. Henikoff,
  • Kami Ahmad,
  • Steven Henikoff

DOI
https://doi.org/10.1038/s41467-019-09982-5
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 10

Abstract

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Understanding gene regulation will require mapping specific chromain features in a small number of cells at high resolution. Here the authors describe CUT&Tag, which uses antibody-mediated tethering of Tn5 transposase to a chromatin protein to generate high resolution libraries.