Emerging Infectious Diseases (Mar 2004)

Immunofluorescence Assay for Serologic Diagnosis of SARS

  • Paul K.S. Chan,
  • King-Cheung Ng,
  • Rickjason C. W. Chan,
  • Rebecca K. Y. Lam,
  • Viola C. Y. Chow,
  • Mamie Hui,
  • Alan Wu,
  • Nelson Lee,
  • Florence H.Y. Yap,
  • Frankie W. T. Cheng,
  • Joseph J. Y. Sung,
  • John S. Tam

DOI
https://doi.org/10.3201/eid1003.030493
Journal volume & issue
Vol. 10, no. 3
pp. 530 – 532

Abstract

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We evaluated a virus-infected cell-based indirect immunofluorescence assay for detecting anti–severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.

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