Cancer Management and Research (May 2019)

Functional variants of lncRNA LINC00673 and gastric cancer susceptibility: a case-control study in a Chinese population

  • Zhao K,
  • Zhang R,
  • Li T,
  • Xiong Z

Journal volume & issue
Vol. Volume 11
pp. 3861 – 3868

Abstract

Read online

Kexin Zhao,1 Rui Zhang,2 Tiantian Li,3 Zhifan Xiong11Division of Gastroenterology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430077, People’s Republic of China; 2The Second Clinical Medical College, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People’s Republic of China; 3College of Life Science & Technology, Huazhong University of Science and Technology, Wuhan 430074, People’s Republic of ChinaBackground: Two genome-wide association studies (GWASs) identified LINC00673 rs11655237 was associated with susceptibility to pancreatic cancer.Methods: To investigate the association between LINC00673 polymorphisms and gastric cancer (GC) risk, and the impact of gene-environmental interaction on GC risk, we conducted this case-control study in a Chinese population.Results: We found rs11655237 significantly increased susceptibility of GC in the Chinese population (OR=1.29; 95% CI=1.12–1.48; P=4.1×10−4,), and a significant interaction was found between rs11655237 and Helicobacter pylori infection (P=0.006). Expression of LINC00673 was significantly higher in adjacent normal tissues than in paired cancer tissues (P<0.001) and significantly lower in the cancer or paired adjacent normal tissues of GC patients with rs11655237 allele A than in those with rs11655237 allele G (P<0.001). Mechanism exploration found that, the construct with the rs11655237[A] allele had significantly reduced luciferase activity in the presence of miR-1231, and this effect could be completely rescued when miR-1231 inhibitor was present.Conclusion: Our results indicate that LINC00673 rs11655237 is associated with an increased GC risk, possibly by down-regulating LINC00673 expression through creating a miR-1231 binding site.Keywords: gastric cancer, LINC00673, genetic, rs11655237, polymorphism

Keywords