Nanotechnology, Science and Applications (Jan 2024)
Factors Affecting the Synthesis of Bovine Serum Albumin Nanoparticles Using the Desolvation Method
Abstract
Yenni Puspita Tanjung,1,2 Mayang Kusuma Dewi,1 Vesara Ardhe Gatera,3,4 Melisa Intan Barliana,3,5 I Made Joni,6,7 Anis Yohana Chaerunisaa1 1Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia; 2Bumi Siliwangi Academy of Pharmacy, Bandung, West Java, Indonesia; 3Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas Padjadjaran, Bandung, Indonesia; 4Department of Pharmacy and Health Sciences, Universiti Kuala Lumpur – Royal College of Medicine Perak, Ipoh, Perak, Malaysia; 5Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia; 6Department of Physics, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Bandung, Indonesia; 7Functional Nano Powder University Center of Excellence (FiNder U CoE), Universitas Padjadjaran, Bandung, IndonesiaCorrespondence: Anis Yohana Chaerunisaa, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km 21, Jatinangor, 45363, Indonesia, Email [email protected]: Currently, protein-based nanoparticles are in high demand as drug delivery systems due to their exceptional qualities, including nontoxicity, nonantigenicity, and biodegradability. Other qualities include high nutritional value, abundance of renewable resources, excellent drug binding capacity, greater stability during storage and in vivo, as well as ease of upgrading during manufacture. Examples of protein suitable for this purpose include ovalbumin (OVA) derived from egg white, human serum albumin (HSA), and bovine serum albumin (BSA). To create albumin nanoparticles, six different processes have been investigated in depth and are frequently used in drug delivery systems. These included desolvation, thermal gelation, emulsification, NAB technology, self-assembly, and nanospray drying. Several experimental conditions in the synthesis of albumin nanoparticles can affect the physicochemical characterization. Therefore, this study aimed to provide an overview of various experimental conditions capable of affecting the physicochemical characteristics of BSA nanoparticles formed using the desolvation method. By considering the variation in optimal experimental conditions, a delivery system of BSA nanoparticles with the best physicochemical characterization results could be developed.Keywords: bovine serum albumin, BSA, desolvation, experimental conditions, physicochemical characteristics
