IN VITRO REPROGRAMMING OF NITRIC OXIDE-SYNTHETIC MECHANISMS FOR M1 AND M2 PHENOTYPES OF MURINE PERITONEAL MACROPHAGES: DEPENDENCE ON DIFFERENT SERUM CONCENTRATIONS

Abstract

Abstract. Macrophages are among key players of inflammatory events, acquiring either pro-inflammatory M1, or anti-inflammatory M2 phenotype, dependent on their microenvironment. Some endogenous macrophagereprogramming factors (MRFs), e.g., TGF-β (an M2-inducing agent), are found in blood serum. This work confirms a hypothesis on in vitro M1-to-M2 phenotype reprogramming of cultured macrophages, by means of decrease or increase in serum concentration. Noteworthy, the macrophages from C57BL/6 и BALB/c mice strains did not differ with their reprogramming capacity. In general, our results allow of extending present knowledge about a role of environmental factors in regulation of macrophage activities.

Keywords

• reprogramming
• macrophages
• phenotype
• nitric oxide