Development of a lyophilized preparation enriched with hepatocyte growth factor

Abstract

The study was performed using neonatal livers from Wistar rats. To obtain cells, it is proposed to use a mixed disaggregation method. A significant increase in the amount of the regulatory peptide on the 5th day was revealed to 46.8 (38.4–50.0) ng/ml, and therefore for lyophilization it is better to use a 5-day cell culture containing the maximum amount of HGF. Optimally selected technological conditions made it possible to preserve biologically active substances secreted by liver cells and obtain a stable form of lyophilized liver cells enriched with hepatocyte growth factor.

Keywords

• lyophilization
• liver cells
• hepatocyte growth factor
• hgf
• liver failure