Nature Communications (Jul 2019)
Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus
- Ian R. Monk,
- Nausad Shaikh,
- Stephanie L. Begg,
- Mike Gajdiss,
- Liam K. R. Sharkey,
- Jean Y. H. Lee,
- Sacha J. Pidot,
- Torsten Seemann,
- Michael Kuiper,
- Brit Winnen,
- Rikki Hvorup,
- Brett M. Collins,
- Gabriele Bierbaum,
- Saumya R. Udagedara,
- Jacqueline R. Morey,
- Neha Pulyani,
- Benjamin P. Howden,
- Megan J. Maher,
- Christopher A. McDevitt,
- Glenn F. King,
- Timothy P. Stinear
Affiliations
- Ian R. Monk
- Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne
- Nausad Shaikh
- Institute for Molecular Bioscience, The University of Queensland
- Stephanie L. Begg
- Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne
- Mike Gajdiss
- University Clinics of Bonn, Institute of Medical Microbiology, Immunology and Parasitology
- Liam K. R. Sharkey
- Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne
- Jean Y. H. Lee
- Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne
- Sacha J. Pidot
- Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne
- Torsten Seemann
- Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne
- Michael Kuiper
- CSIRO Data61
- Brit Winnen
- Rikki Hvorup
- Institute for Molecular Bioscience, The University of Queensland
- Brett M. Collins
- Institute for Molecular Bioscience, The University of Queensland
- Gabriele Bierbaum
- University Clinics of Bonn, Institute of Medical Microbiology, Immunology and Parasitology
- Saumya R. Udagedara
- Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University
- Jacqueline R. Morey
- Department of Molecular and Biomedical Sciences, School of Biological Sciences, The University of Adelaide
- Neha Pulyani
- Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University
- Benjamin P. Howden
- Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne
- Megan J. Maher
- Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University
- Christopher A. McDevitt
- Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne
- Glenn F. King
- Institute for Molecular Bioscience, The University of Queensland
- Timothy P. Stinear
- Department of Microbiology and Immunology, Doherty Institute for Infection and Immunity, University of Melbourne
- DOI
- https://doi.org/10.1038/s41467-019-10932-4
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 13
Abstract
WalKR is an essential two-component regulator that controls peptidoglycan synthesis in the human pathogen Staphylococcus aureus. Here, the authors provide biochemical, structural, and functional evidence supporting that the binding of a zinc ion inhibits autophosphorylation and thus alters WalKR regulatory activity.
