A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol

Christoph Gerdes; Natalia Waal; Thomas Offner; Eugenio F. Fornasiero; Nora Wender; Hannes Verbarg; Ivan Manzini; Claudia Trenkwalder; Brit Mollenhauer; Timo Strohäker; Markus Zweckstetter; Stefan Becker; Silvio O. Rizzoli; Fitnat Buket Basmanav; Felipe Opazo

doi:10.1038/s41467-020-16575-0

Nature Communications (Jun 2020)

A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol

Christoph Gerdes,
Natalia Waal,
Thomas Offner,
Eugenio F. Fornasiero,
Nora Wender,
Hannes Verbarg,
Ivan Manzini,
Claudia Trenkwalder,
Brit Mollenhauer,
Timo Strohäker,
Markus Zweckstetter,
Stefan Becker,
Silvio O. Rizzoli,
Fitnat Buket Basmanav,
Felipe Opazo

Affiliations

Christoph Gerdes: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen
Natalia Waal: Center for Biostructural Imaging of Neurodegeneration (BIN), University Medical Center Göttingen
Thomas Offner: Institute of Animal Physiology, Department of Animal Physiology and Molecular Biomedicine, Justus-Liebig University Giessen
Eugenio F. Fornasiero: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen
Nora Wender: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen
Hannes Verbarg: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen
Ivan Manzini: Institute of Animal Physiology, Department of Animal Physiology and Molecular Biomedicine, Justus-Liebig University Giessen
Claudia Trenkwalder: Department of Neurosurgery, University Medical Center Göttingen
Brit Mollenhauer: Paracelsus-Elena-Klinik, Klinikstraße 16
Timo Strohäker: German Center for Neurodegenerative Diseases (DZNE), Von-Siebold-Str. 3a
Markus Zweckstetter: German Center for Neurodegenerative Diseases (DZNE), Von-Siebold-Str. 3a
Stefan Becker: Department for NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry
Silvio O. Rizzoli: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen
Fitnat Buket Basmanav: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen
Felipe Opazo: Department of Neuro- and Sensory Physiology, University Medical Center Göttingen

DOI: https://doi.org/10.1038/s41467-020-16575-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 13

Abstract

Read online

α-Syn in CSF is a biomarker of neurodegenerative diseases; however, the detection of clinically relevant species is difficult. Here, the authors create a nanobody biosensor that reveals the presence of α-Syn in cells, which allow the detection of transmittable forms of α-Syn present in human CSF.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal