Ultrasensitive dynamic light scattering immunosensing platform for NT-proBNP detection using boronate affinity amplification

Jiaqi Hu; Lu Ding; Jing Chen; Jinhua Fu; Kang Zhu; Qian Guo; Xiaolin Huang; Yonghua Xiong

doi:10.1186/s12951-021-01224-5

Journal of Nanobiotechnology (Jan 2022)

Ultrasensitive dynamic light scattering immunosensing platform for NT-proBNP detection using boronate affinity amplification

Jiaqi Hu,
Lu Ding,
Jing Chen,
Jinhua Fu,
Kang Zhu,
Qian Guo,
Xiaolin Huang,
Yonghua Xiong

Affiliations

Jiaqi Hu: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University
Lu Ding: Hypertension Research Institute of Jiangxi Province, Department of Cardiology, The First Affiliated Hospital of Nanchang University
Jing Chen: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University
Jinhua Fu: Jiangxi Agricultural Technology Extension Center
Kang Zhu: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University
Qian Guo: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University
Xiaolin Huang: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University
Yonghua Xiong: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University

DOI: https://doi.org/10.1186/s12951-021-01224-5
Journal volume & issue: Vol. 20, no. 1
pp. 1 – 10

Abstract

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Highlight 1. We developed a new DLS immunosensing technology for the rapid and sensitive detection of glycoprotein NT-proBNP. 2. The boronate affinity recognition amplified nanoparticle aggregation was designed to enable highly sensitive DLS transduction. 3. The fabricated DLS immunosensor exhibited ultrahigh sensitivity with an LOD of 7.4 fg mL−1, fast response time (< 20 min), and small sample consumption (1 μL). 4. This boronate affinity amplified-DLS immunosensor has broad prospects for field or point-of-care testing of cis-diol-containing molecules.

Published in Journal of Nanobiotechnology

ISSN: 1477-3155 (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Technology: Chemical technology: Biotechnology; Medicine: Medicine (General): Medical technology
Website: https://jnanobiotechnology.biomedcentral.com/

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Abstract

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