International Journal of Nanomedicine (Jun 2021)
Targeting Antibacterial Effect and Promoting of Skin Wound Healing After Infected with Methicillin-Resistant Staphylococcus aureus for the Novel Polyvinyl Alcohol Nanoparticles
Abstract
Dengyan Wu,1 Dong Wei,2 Maotao Du,1 Song Ming,1 Qian Ding,1 Ranjing Tan1 1Department of Dermatology, Second affiliated Hospital, Chongqing Medical University, Chongqing, 400010, People’s Republic of China; 2Plastic Surgery, Pengshui County People’s Hospital, Pengshui, 409600, People’s Republic of ChinaCorrespondence: Ranjing TanDepartment of Dermatology, Second affiliated Hospital, Chongqing Medical University, Chongqing, 400010, People’s Republic of ChinaEmail [email protected]: Topical agents typically remain in the wound site for time duration that are too short to effectively eradicate MRSA tradition formation of BZK that can be maintained within the wound site for longer time periods, should be more effective.Methods: The novel chitosan and poly (D,L-lactide-co-glycoside) nanoparticles loaded with benzalkonium bromide (BZK) were designed, for the promotion wound healing after MRSA infection. The physical characterization of these nanoparticles, as well as their antibacterial activity in vitro, release profile in simulated wound fluid, cell toxicity, anti-biofilm activity, and their ability to improve the skin wound healing in a mouse model were also studied.Results: These novel nanoparticles were found to have a significant antibacterial activity (p< 0.01), both in vitro and in vivo test. The stronger anti-biofilm ability of the nanoparticles to inhibit the formation of bacterial biofilms, at a concentration of 3.33 μg/mL, and clear existing bacterial biofilms, at a concentration of 5 mg/mL, compared with its water solution. In addition, significant damage to bacterial cell walls also was found, providing insight into the mechanism of antibacterial activity.Conclusion: Taken together, these results demonstrated the ability of BZK-loaded nanoparticles in the promotion of skin wound healing with MRSA infection. The current findings open a new avenue for nanomedicine development and future clinical applications in the treatment of wounds.Keywords: MRSA, wound, nanoparticles, antibacterial effect, biofilm
