In Vitro Investigation of the Effect of Different Mouthwashes Applied to Restorative Dental Materials on Adhesion of Streptococus mutans

Abstract

Objective:This study aims to evaluate the effect of chlorhexidine gluconate (CHX), Listerine, and boric acid (BA), applied to three different restorative dental materials, on Streptococcus mutans (S. mutans) adhesion.Methods:A total of 120 samples were prepared in the study: Composite (Group CR; n=40), glass ionomer cement (Group GIC; n=40) and compomer (Group C; n=40). The upper and lower surface roughness of the samples were measured. After bacterial adhesions, each group was separated into four subgroups (n=10). Three mouthwash and distilled water (DS) were applied for one minute. Subsequently, the remaining S. mutans biofilms were examined by colony forming unit count (CFU) and MTT methods. Data were evaluated (p<0.05).Results:The GIC was the highest, and CR was the lowest in terms of surface roughness. There was a difference among three groups (p<0.001). Log CFU efficiency of bacterial counts of all 3 mouthwashes was higher than distilled water (p<0.001). The effect of all three mouthwash and DW on S. mutans log cfu and MTT values differed between the groups (p<0.001). CHX was the most effective. In terms of restorative material-mouthwash interactions, differences were found between the groups (p<0.05). There was a positive moderate statistically significant correlation between log cfu and MTT values (r=0.636; p<0.001).Conclusion:BA can be an alternative to other mouthwashes due to its natural structure and the minimal side effects.

Keywords

• bacterial adhesion
• restorative dental material
• restorative dental treatment
• colony forming unit