Unexpected consequences: exon skipping caused by CRISPR-generated mutations

Abstract

Abstract A new study finds that splicing disruption is a frequent consequence of mutations generated by CRISPR/Cas9 gene-editing technology, and alleles designed to be null can express aberrant proteins. This new information allows enhanced quality control procedures to select the best mutant alleles generated by CRISPR/Cas9. Please see related Method article: https://www.doi.org/10.1186/s13059-017-1237-8