Establishment human induced pluripotent stem cell line from idiopathic non-familial Parkinson’s disease patient using self-replicating RNA vector

Harry Murti; Kelvin Pieknell; Indra Bachtiar; Siska Yuliana Sari; Yanuar Alan Sulistio; Sang-Hun Lee

Stem Cell Research (Jan 2021)

Establishment human induced pluripotent stem cell line from idiopathic non-familial Parkinson’s disease patient using self-replicating RNA vector

Harry Murti,
Kelvin Pieknell,
Indra Bachtiar,
Siska Yuliana Sari,
Yanuar Alan Sulistio,
Sang-Hun Lee

Affiliations

Harry Murti: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia
Kelvin Pieknell: Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul, Republic of Korea; Hanyang Biomedical Research Institute, Hanyang University, Seoul, Republic of Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Republic of Korea
Indra Bachtiar: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia
Siska Yuliana Sari: Department of Translational Research Stem Cell, Stem Cell and Cancer Institute, PT. Kalbe Farma Tbk., Jakarta, Indonesia; ReGeniC Laboratory, PT. Bifarma Adiluhung, Jakarta, Indonesia
Yanuar Alan Sulistio: Hanyang Biomedical Research Institute, Hanyang University, Seoul, Republic of Korea; Corresponding author at: Hanyang Biomedical Research Institute, Hanyang University, Seoul, Republic of Korea.
Sang-Hun Lee: Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, Seoul, Republic of Korea; Hanyang Biomedical Research Institute, Hanyang University, Seoul, Republic of Korea; Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Republic of Korea

Journal volume & issue: Vol. 50
p. 102137

Abstract

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Induced pluripotent stem cell (iPSC) line HUi002-A was reprogrammed from skin fibroblasts via non-integrating, virus free self-replicating RNA. Skin fibroblasts from a 53-year-old male Caucasian, non-familial Parkinson's disease patient, idiopathic (clinical summary confirmed Parkinson's disease) was obtained from the Coriell Institute (AG20442). Generated iPSCs were characterized and pluripotency was confirmed.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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