Synthetic energy sensor AMPfret deciphers adenylate-dependent AMPK activation mechanism

Martin Pelosse; Cécile Cottet-Rousselle; Cécile M. Bidan; Aurélie Dupont; Kapil Gupta; Imre Berger; Uwe Schlattner

doi:10.1038/s41467-019-08938-z

Nature Communications (Mar 2019)

Synthetic energy sensor AMPfret deciphers adenylate-dependent AMPK activation mechanism

Martin Pelosse,
Cécile Cottet-Rousselle,
Cécile M. Bidan,
Aurélie Dupont,
Kapil Gupta,
Imre Berger,
Uwe Schlattner

Affiliations

Martin Pelosse: University of Grenoble Alpes and INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA) and SFR Environmental and Systems Biology (BEeSy), Rue de la Piscine, Domaine Universitaire
Cécile Cottet-Rousselle: University of Grenoble Alpes and INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA) and SFR Environmental and Systems Biology (BEeSy), Rue de la Piscine, Domaine Universitaire
Cécile M. Bidan: University of Grenoble Alpes, CNRS, Laboratoire Interdisciplinaire de Physique (LIPhy)
Aurélie Dupont: University of Grenoble Alpes, CNRS, Laboratoire Interdisciplinaire de Physique (LIPhy)
Kapil Gupta: Bristol Synthetic Biology Centre BrisSynBio, Biomedical Sciences, University of Bristol
Imre Berger: Bristol Synthetic Biology Centre BrisSynBio, Biomedical Sciences, University of Bristol
Uwe Schlattner: University of Grenoble Alpes and INSERM U1055, Laboratory of Fundamental and Applied Bioenergetics (LBFA) and SFR Environmental and Systems Biology (BEeSy), Rue de la Piscine, Domaine Universitaire

DOI: https://doi.org/10.1038/s41467-019-08938-z
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

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AMP-activated protein kinase AMPK senses and regulates cellular energy state. Here the authors engineer a synthetic sensor, AMPfret, that allows direct, real-time readout of the AMPK conformational state by fluorescence resonance energy transfer (FRET).

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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