Anti-nociceptive Effect of Entada Phaseoloides Seeds Formulation after Topical Application in Arthritic Wistar Rats

Abstract

Objectives: To study the effect of topical application of different formulations of Entada phaseoloides seeds for analgesic activity in arthritic wistar rats. Material and Methods: EP (Entada Phaseoloides) seeds were powdered after removing the shell. Paste was prepared by adding water and ointment with polyethylene glycol (PEG 400) & Carbowax 3350. 32 Wister rats of either sex weighing 150- 200 gms were divided into four groups ,Gr-I vehicle, Gr-II EP paste, Gr-III EP Ointment, Gr-IV Diclofenac Sodium Ointment. For induction of arthritis 0.1ml Complete Freund’s Adjuvant (CFA) was given by intra-articular injection in left knee joint. Drug treatment was started on the same day by topical application in all groups respectively. Pain threshold for referred pain was measured on Randall Selitto analgesymeter before and after drug treatment. Results: At 0 hours pain threshold of animals from all the groups was approximately similar. Pain threshold in control group was statistically significantly decreased at 72 hours. In comparison with control there was significant (p<0.001) increase in the pain threshold in the EP treated groups after 72 hours. Conclusion: Both the formulations of EP have analgesic activity comparable to Diclofenac sodium.

Keywords

• complete freund’s adjuvant
• randall selitto analgesymeter
• analgesic