EFFECT OF REDOX POTENTIALS ON PHYSIOLOGICAL STATE OF MICROORGANISMS OF GENUS CLOSTRIDIUM

Abstract

The effect of redox potential (ORP) of the medium on formation of biomass and metabolic products by anaerobic thermophilic (60 °C) microorganisms of genus Clostridium: cellulolytic – C. thermocellum 5ST and saccharolytic C. thermo-saccharolyticum 1S under anaerobic conditions. There were shown, that strains development takes place in the various limits and within the optimum ORP. Growth of C. thermocellum 5CT is observed within ORP -200 ...- 380 mV, with an optimum -260 mV; C. thermosaccharolyticum 1S in the range redox potential +100 ..- 200 mV, with an optimum -135 ...- 140 mV (pH 7,0–7,3). For optimum values of redox potential in both strains, biomass growth, ethanol and acetate in formation medium with a reducing agent (sodium sulfide) in 1.5–2 times higher than that without a reducing agent. There were determined that the redox potential is one of the main factors determining the physiological state of the cells and biosynthesis intensity.

Keywords

• окисно-відновний потенціал
• рн
• біомаса
• clostrіdіum thermocellum
• c. thermosaccharolyticum
• продукція
• етанол
• ацетат
• н2