An optimized protocol for phenotyping human granulocytes by mass cytometry

Nora Vivanco Gonzalez; John-Paul Oliveria; Dmitry Tebaykin; Geoffrey T. Ivison; Kaori Mukai; Mindy M. Tsai; Luciene Borges; Kari C. Nadeau; Stephen J. Galli; Albert G. Tsai; Sean C. Bendall

STAR Protocols (Jun 2022)

An optimized protocol for phenotyping human granulocytes by mass cytometry

Nora Vivanco Gonzalez,
John-Paul Oliveria,
Dmitry Tebaykin,
Geoffrey T. Ivison,
Kaori Mukai,
Mindy M. Tsai,
Luciene Borges,
Kari C. Nadeau,
Stephen J. Galli,
Albert G. Tsai,
Sean C. Bendall

Affiliations

Nora Vivanco Gonzalez: Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States; Corresponding author
John-Paul Oliveria: Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States; Department of Medicine, Division of Respirology, McMaster University, Hamilton, ON, L8S4K1, Canada
Dmitry Tebaykin: Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States
Geoffrey T. Ivison: Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States
Kaori Mukai: Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States; Sean N. Parker Center for Allergy Research, School of Medicine, Stanford University, Stanford, CA, United States
Mindy M. Tsai: Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States; Sean N. Parker Center for Allergy Research, School of Medicine, Stanford University, Stanford, CA, United States
Luciene Borges: Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States
Kari C. Nadeau: Sean N. Parker Center for Allergy Research, School of Medicine, Stanford University, Stanford, CA, United States; Department of Medicine, Division of Pulmonary and Critical Care Medicine, School of Medicine, Stanford University, Stanford, CA, United States
Stephen J. Galli: Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States; Sean N. Parker Center for Allergy Research, School of Medicine, Stanford University, Stanford, CA, United States; Department of Microbiology and Immunology, School of Medicine, Stanford University, Stanford, CA, United States
Albert G. Tsai: Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States
Sean C. Bendall: Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States; Corresponding author

Journal volume & issue: Vol. 3, no. 2
p. 101280

Abstract

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Summary: Granulocytes encompass diverse roles, from fighting off pathogens to regulating inflammatory processes in allergies. These roles are represented by distinct cellular phenotypes that we captured with mass cytometry (CyTOF). Our protocol enables simultaneous evaluation of human basophils, eosinophils, and neutrophils under homeostasis and upon immune activation by anti-Immunoglobulin E (anti-IgE) or interleukin-3 (IL-3). Granulocyte integrity and detection of protein markers were optimized so that rare granulocyte populations could be deeply characterized by single cell mass cytometry.For complete details on the use and execution of this protocol, please refer to Vivanco Gonzalez et al. (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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