Molecular Analysis of mps Operon Involved in Monapterin Syntliesis in Escherichia Coli

Abstract

Two genes, mpsA and mpsB, constitute an operon in the synthesis of monapterin in E. coli. Expression of mpsA, the structural gene for dihydroneopterin triphosphate 2'-epimerase, from its native promoter, required mpsB. Cotransfonnation of mpsB excludes the possibility that the MpsB is a transactivator protein for mpsA expression.

Keywords

• monapterin
• l-threo-neopterin
• mps operon
• dihydroneopterin triphosphate 2'-epimerasc