Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP

Yaxiong Yang; Nan Liu; Yuanyuan He; Yuxia Liu; Lin Ge; Linzhi Zou; Sen Song; Wei Xiong; Xiaodong Liu

doi:10.1038/s41467-018-03719-6

Nature Communications (Apr 2018)

Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP

Yaxiong Yang,
Nan Liu,
Yuanyuan He,
Yuxia Liu,
Lin Ge,
Linzhi Zou,
Sen Song,
Wei Xiong,
Xiaodong Liu

Affiliations

Yaxiong Yang: Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University
Nan Liu: Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University
Yuanyuan He: Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University
Yuxia Liu: Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University
Lin Ge: Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University
Linzhi Zou: School of Life Sciences, Tsinghua University
Sen Song: Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University
Wei Xiong: IDG/McGovern Institute for Brain Research, Tsinghua University
Xiaodong Liu: Department of Biomedical Engineering, School of Medicine, X-Lab for Transmembrane Signaling Research, Tsinghua University

DOI: https://doi.org/10.1038/s41467-018-03719-6
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 18

Abstract

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The popular genetically-encoded Ca2+ indicator, GCaMP, has several side-effects. Here the authors show that GCaMP containing CaM interferes with gating and signaling of L-type calcium channels, which disrupts Ca2+ dynamics and gene expression, and develop GCaMP-X to overcome these limitations.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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