Journal of Medicinal Plants (Aug 2008)
Comparision of MICs of some Iranian Herbal Essences Against Azole Resistance and Azole Susceptible of Candida Albicans
Abstract
Background: Candida albicans is the most important species, cause candida infections. The common forms of candidiasis are cutaneous and mucosal candidiasis, include oral (thrush), Vaginal candidiasis and onychomycosis. Different studies showed the drug resistance between candida albicans isolates, so it seems necessary to find replacement treatment for prevention of this resistance, side effects and avoidance of recurrent infection. Objective: Aim of this study is to determine of MICs of three essences against candida albicans isolates. Also, we compared the effects of essences between azole resistance and azole susceptible isolates. Method: In this study, 30 candida albicans isolates (16 azole resistance and 14 azole susceptible isolates) obtained from oral, vaginal and nails of patients with candidiasis were evaluated. In order to determine of susceptibility of Zataria multiflora, Geranium herbarum and Artemisia sieberi essences against candida albicans, Macrodilution broth Serial dilution of essences was prepared in sabouraud glucose broth tubes. Essences were solved in DMSO (0.12) as solvent. The same solvent at an appropriate concentration was used as a control (with and without organisms). After inoculation, incubation and subculture, MICs, compared to control tubes were determined. Results: The obtained results of MICs for Zataria multiflora, Geranium herbarum and Artemisia sieberi were between 0.11-0.25 µl/ml (mean: 0.18µl/ml), 0.4-1.25µl/ml (mean: 0.83µl/ml) and 0.62-1.25 µl/ml (mean: 1.4µl/ml), respectively. Also, there are not significant differences in MICs of these essences against azole resistance and azole susceptible isolates. Conclusion: We conclude that anti-candida activity of three herbal essences under study, against azole resistance and azole susceptible isolates were similar. With attention to resistance of different candida albicans to synthetic and chemical antifungals, these herbal essences are safe replacement treatments for cutaneous and mucosal candidia infections.
