LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark

Muhamad Salman Fareza; Nur Amalia  Choironi; Sri Sutji  Susilowati; Melina Puspita  Rini; Vyola  Festihawa; Irfansyah Septian Nur  Fauzi; Esti Dyah  Utami; Sarmoko Sarmoko

doi:10.22146/ijp.1328

Indonesian Journal of Pharmacy (Sep 2021)

LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark

Muhamad Salman Fareza,
Nur Amalia Choironi,
Sri Sutji Susilowati,
Melina Puspita Rini,
Vyola Festihawa,
Irfansyah Septian Nur Fauzi,
Esti Dyah Utami,
Sarmoko Sarmoko

Affiliations

Muhamad Salman Fareza: Jurusan Farmasi, Universitas Jenderal Soedirman. Purwokerto
Nur Amalia Choironi: Pharmaceutical Biology Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Sri Sutji Susilowati: Pharmaceutical Chemistry Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Melina Puspita Rini: Pharmaceutical Chemistry Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Vyola Festihawa: 2. Pharmaceutical Biology Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Irfansyah Septian Nur Fauzi: 1. Pharmaceutical Chemistry Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Esti Dyah Utami: Pharmacology and Clinical Pharmacy Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123
Sarmoko Sarmoko: Pharmacology and Clinical Pharmacy Laboratory, Department of Pharmacy, Jendral Soedirman University, Karangwangkal Purwokerto Campus, Indonesia, 53123

DOI: https://doi.org/10.22146/ijp.1328

Abstract

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Calophyllum soulattri (Sulatri), a plant from Clusiaceae family, has been empirically used as traditional medicine. In the present study, C. soulattri stem bark extract and fractions were evaluated for their toxicity against MCF-7 breast cancer cell. The extract and fraction’s chemical content was analyzed using the combination of liquid chromatography with mass spectrometry (LC-MS/MS). The results showed that methanol extract and n-hexane fractions have strong cytotoxic activity with IC50 values 93.6 and 36 µg/mL, respectively. Meanwhile, as the positive control, ethyl acetate and cisplatin fractions have IC50 values 233 and 16.2 µg/mL, respectively. The LC-MS/MS analysis showed that the extract and fractions contained polyporusterone A, poricoic acid D, polyporusterone F, esulentagenin, and 1-acetyl-3-(methoxy-carbonyl)-β-carboline. Therefore, C. soulattri stem bark extract and fractions have potential activity as an anticancer agent that was able to inhibit MCF-7 breast cancer cell growth.

callophylum soulattri, stem bark, lc-ms/ms analysis, mcf-7

Published in Indonesian Journal of Pharmacy

ISSN: 2338-9427 (Print); 2338-9486 (Online)
Publisher: Universitas Gadjah Mada
Country of publisher: Indonesia
LCC subjects: Medicine: Pharmacy and materia medica
Website: https://jurnal.ugm.ac.id/v3/IJP

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