Data in support of DPF2 regulates OCT4 protein level and nuclear distribution

Chao Liu; Dijuan Zhang; Yuxian Shen; Xiaofang Tao; Lihua Liu; Yongwang Zhong; Shengyun Fang

doi:10.1016/j.dib.2015.10.010

Data in Brief (Dec 2015)

Data in support of DPF2 regulates OCT4 protein level and nuclear distribution

Chao Liu,
Dijuan Zhang,
Yuxian Shen,
Xiaofang Tao,
Lihua Liu,
Yongwang Zhong,
Shengyun Fang

Affiliations

Chao Liu: Department of Histology and Embryology, Institute of Stem Cell and Tissue Engineering, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, China
Dijuan Zhang: Department of Histology and Embryology, Institute of Stem Cell and Tissue Engineering, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui 230032, China
Yuxian Shen: School of Basic Medical Sciences, Institute of Biopharmaceuticals, Anhui Medical University, Hefei, Anhui 230032, China
Xiaofang Tao: School of Basic Medical Sciences, Institute of Biopharmaceuticals, Anhui Medical University, Hefei, Anhui 230032, China
Lihua Liu: Institute of Clinical Pharmacology, Anhui Medical University, Hefei, Anhui 230032, China
Yongwang Zhong: Center for Biomedical Engineering and Technology (BioMET), University of Maryland, Baltimore, MD 21201, USA
Shengyun Fang: Center for Biomedical Engineering and Technology (BioMET), University of Maryland, Baltimore, MD 21201, USA

DOI: https://doi.org/10.1016/j.dib.2015.10.010
Journal volume & issue: Vol. 5, no. C
pp. 599 – 604

Abstract

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DPF2, also named ubi-d4/requiem (REQU), interacts with a protein complex containing OCT4. This paper provides data in support of the research article entitled “DPF2 regulates OCT4 protein level and nuclear distribution”. The highlights include: (1) Denature-immunoprecipitation assay revealed ubiquitination of OCT4 in pluripotent H9 cells, which was enhancedby MG132, a proteasome inhibitor. (2) Well colocalization of ectopic OCT4 and FLAG-Ub was found in HeLa cells, which was also increased by MG132. (3) MG132 treatment decreased DPF2 cytoplasmic expression in vivo. These data give insights into how proteasome inhibition contributes to studying ubiquitnation of OCT4.

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

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