X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs

Qinan Hu; Olga A. Antipova; Thomas V. O’Halloran; Mariana F. Wolfner

STAR Protocols (Mar 2021)

X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs

Qinan Hu,
Olga A. Antipova,
Thomas V. O’Halloran,
Mariana F. Wolfner

Affiliations

Qinan Hu: Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
Olga A. Antipova: X-ray Sciences Division, Argonne National Laboratory, Argonne, IL 60439, USA
Thomas V. O’Halloran: The Chemistry of Life Processes Institute, Northwestern University, Evanston, IL 60208, USA; Department of Chemistry, Northwestern University, Evanston, IL 60208, USA; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA
Mariana F. Wolfner: Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 1
p. 100247

Abstract

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Summary: X-ray fluorescence microscopy (XFM) is a powerful tool for mapping and quantifying the spatial distribution of elemental composition of biological samples. Recently, it was reported that transition metal fluctuations occur during Drosophila reproduction, analogous to what is seen in mammals and nematodes, and may contribute to Drosophila female fertility. To further support XFM studies on Drosophila reproduction, we describe procedures for isolating oocytes and activated eggs and examining their elemental composition by XFM scanning and analysis.For complete details on the use and execution of this protocol, please refer to Hu et al. (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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