Pectin mediated green synthesis of Fe3O4/Pectin nanoparticles under ultrasound condition as an anti-human colorectal carcinoma bionanocomposite

Abstract

This work described the one-pot synthesis of apple pectin encapsulated Fe3O4 nanoparticles (Fe3O4/Pectin NPs) which is prepared by co-precipitation of Fe(II/(III) ions in alkaline solution mediated by pectin under ultrasound condition. This process led to formation of magnetic nanoparticles within the network of pectin. Physicochemical characterization of the as-synthesized Fe3O4/Pectin NPs was carried out through electron microscopy (SEM and TEM), energy dispersive X-ray spectroscopy (EDX), vibrating sample magnetometer (VSM) and X-ray diffraction (XRD). The in vitro cytotoxic and anti-colorectal cancer effects of biologically synthesized Fe3O4/Pectin NPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29 cancer cell lines were assessed. The anti-colorectal cancer properties of the Fe3O4/Pectin NPs could significantly remove Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29 cancer cell lines in a time and concentration-dependent manner by MTT assay. The IC50 of the Fe3O4/Pectin NPs were 317, 337, 187, and 300 µg/mL against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29 cancer cell lines. The antioxidant activity of Fe3O4/Pectin NPs was determined by DPPH method. The Fe3O4/Pectin NPs showed the high antioxidant activity according to the IC50 value. It seems that the anti-human colorectal cancer effect of recent nanoparticles is due to their antioxidant effects.

Keywords

• Pectin
• Magnetic
• Nanocomposite
• Cytotoxicity
• Antioxidant
• Anti-colorectal cancer