Generating tetraploid zebrafish by heat shock treatment and labeling microtubules of their cells in vitro

Abstract

Tetraploid is a very important experimental material, whether in scientific research or breeding practice. However, tetraploid strains are relatively rare. It was reported that chromosomes allocation disorder during cell division may lead to high embryonic mortality and low fertility in tetraploid fish. In this paper, tetraploid zebrafish is prepared by ploidy operation. And successfully establishes a tetraploid zebrafish cell line in vitro. To tracing status of microtubules, the tetraploid zebrafish cells are transfected with two plasmids of pBLK-α-tubulin-mCherry and pBLK-γ-tubulin-EGFP by electroporation in vitro. Observation shows that these transfected cells can be traced the changes of spindle and centrosomes during cell division in tetraploid cells. The results provide important material platform in vitro for studying the chromosome behavior in tetraploid cell proliferation, especially the formation mechanism of aneuploidy.

Keywords

• Tetraploid
• Zebrafish
• Tubulin
• Chromosome behavior
• Electroporation