Scientific Papers Animal Science and Biotechnologies (Oct 2023)
GENETICAL INACTIVATION OF PIKEPERCH (SANDER LUCIOPERCA) SPERM USING UV IRRADIATION
Abstract
Although pikeperch is a promising species for intensive aquaculture and some experiments regarding all-female production in this species were made, there are no specifically data regarding genetical inactivation of pikeperch sperm. The aim of this study is to test two different UV lights (15 and 30 Watts) and exposure times of the diluted milt at UV irradiation for sperm genetical inactivation, in order to establish the first step in the gynogenesis protocol for pikeperch. The milt collected from 4 clinically health adult pikepearch males (3-4 years old) was diluted 1:9 with Ringer solution, after that being placed into Petri dishes (Ø = 3 cm) in a thin layer of 1 mm. Two UV lights (λ = 253.7 nm) at 15 Watts (variant V1) and 30 Watts (variant V2) were used in our experiments. These were placed 20 cm above Petri dishes, and were established for each variant, 5 different periods for exposure to the irradiation: 8 min. (T1), 11 min. (T2), 14 min. (T3), 17 min. (T4) and 20 min. (T5). The successful of irradiation was assessed by percent of fertilization (at 2 hours post fertilization), daily percent of survival, percent of hatching and percent of haploids (embryos/larvae with severely curved backbones and other deformities). After this study we could conclude that genetically inactivation of the pikeperch sperm can be performed with 15 Watts and 30 Watts UV lights at an exposure time which can vary between 8 and 20 minutes.
