Proteomic Profiling of Thyroid Papillary Carcinoma

Yoshiyuki Ban; Gou Yamamoto; Michiya Takada; Shigeo Hayashi; Yoshio Ban; Kazuo Shimizu; Haruki Akasu; Takehito Igarashi; Yasuhiko Bando; Tetsuhiko Tachikawa; Tsutomu Hirano

doi:10.1155/2012/815079

Journal of Thyroid Research (Jan 2012)

Proteomic Profiling of Thyroid Papillary Carcinoma

Yoshiyuki Ban,
Gou Yamamoto,
Michiya Takada,
Shigeo Hayashi,
Yoshio Ban,
Kazuo Shimizu,
Haruki Akasu,
Takehito Igarashi,
Yasuhiko Bando,
Tetsuhiko Tachikawa,
Tsutomu Hirano

Affiliations

Yoshiyuki Ban: Division of Diabetes, Metabolism and Endocrinology, Department of Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan
Gou Yamamoto: Department of Oral Pathology and Diagnosis, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
Michiya Takada: Division of Diabetes, Metabolism and Endocrinology, Department of Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan
Shigeo Hayashi: Department of Oral Pathology and Diagnosis, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
Yoshio Ban: Ban Thyroid Clinic, 2-11-16 Jiyugaoka, Megro-ku, Tokyo 152-0035, Japan
Kazuo Shimizu: Division of Endocrine Surgery, Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
Haruki Akasu: Division of Endocrine Surgery, Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
Takehito Igarashi: Division of Endocrine Surgery, Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan
Yasuhiko Bando: Biosys Technologies, Inc., 2-13-18 Nakane, Meguro-ku, Tokyo 152-0031, Japan
Tetsuhiko Tachikawa: Department of Oral Pathology and Diagnosis, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan
Tsutomu Hirano: Division of Diabetes, Metabolism and Endocrinology, Department of Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan

DOI: https://doi.org/10.1155/2012/815079
Journal volume & issue: Vol. 2012

Abstract

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Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy. We performed shotgun liquid chromatography (LC)/tandem mass spectrometry (MS/MS) analysis on pooled protein extracts from patients with PTC and compared the results with those from normal thyroid tissue validated by real-time (RT) PCR and immunohistochemistry (IHC). We detected 524 types of protein in PTC and 432 in normal thyroid gland. Among these proteins, 145 were specific to PTC and 53 were specific to normal thyroid gland. We have also identified two important new markers, nephronectin (NPNT) and malectin (MLEC). Reproducibility was confirmed with several known markers, but the one of two new candidate markers such as MLEC did not show large variations in expression levels. Furthermore, IHC confirmed the overexpression of both those markers in PTCs compared with normal surrounding tissues. Our protein data suggest that NPNT and MLEC could be a characteristic marker for PTC.

Published in Journal of Thyroid Research

ISSN: 2090-8067 (Print); 2042-0072 (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the endocrine glands. Clinical endocrinology
Website: https://www.hindawi.com/journals/jtr/

About the journal