Nature Communications (Feb 2023)
Tandemly duplicated CYP82Ds catalyze 14-hydroxylation in triptolide biosynthesis and precursor production in Saccharomyces cerevisiae
- Yifeng Zhang,
- Jie Gao,
- Lin Ma,
- Lichan Tu,
- Tianyuan Hu,
- Xiaoyi Wu,
- Ping Su,
- Yujun Zhao,
- Yuan Liu,
- Dan Li,
- Jiawei Zhou,
- Yan Yin,
- Yuru Tong,
- Huan Zhao,
- Yun Lu,
- Jiadian Wang,
- Wei Gao,
- Luqi Huang
Affiliations
- Yifeng Zhang
- State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences
- Jie Gao
- State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences
- Lin Ma
- School of Traditional Chinese Medicine, Capital Medical University
- Lichan Tu
- Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province, School of Medicine, Zhejiang University City College
- Tianyuan Hu
- School of Pharmacy, Hangzhou Normal University
- Xiaoyi Wu
- School of Traditional Chinese Medicine, Capital Medical University
- Ping Su
- State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences
- Yujun Zhao
- State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences
- Yuan Liu
- School of Traditional Chinese Medicine, Capital Medical University
- Dan Li
- School of Pharmaceutical Sciences, Capital Medical University
- Jiawei Zhou
- College of Biotechnology and Bioengineering, Zhejiang University of Technology
- Yan Yin
- School of Traditional Chinese Medicine, Capital Medical University
- Yuru Tong
- School of Pharmaceutical Sciences, Capital Medical University
- Huan Zhao
- School of Traditional Chinese Medicine, Capital Medical University
- Yun Lu
- School of Traditional Chinese Medicine, Capital Medical University
- Jiadian Wang
- School of Traditional Chinese Medicine, Capital Medical University
- Wei Gao
- School of Traditional Chinese Medicine, Capital Medical University
- Luqi Huang
- State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences
- DOI
- https://doi.org/10.1038/s41467-023-36353-y
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 14
Abstract
Hydroxylation at the C-14 position of triptolide is critical for its potent antitumor activity. Here, the authors report two CYP82Ds catalyze the 14-hydroxylation reaction via metabolic grid and achieve heterologous bioproduction of triptolide precursor in engineered Saccharomyces cerevisiae.
