<i>Cannabis sativa</i> L. Inflorescences from Monoecious Cultivars Grown in Central Italy: An Untargeted Chemical Characterization from Early Flowering to Ripening

Cinzia Ingallina; Anatoly P. Sobolev; Simone Circi; Mattia Spano; Caterina Fraschetti; Antonello Filippi; Antonella Di Sotto; Silvia Di Giacomo; Giulia Mazzoccanti; Francesco Gasparrini; Deborah Quaglio; Enio Campiglia; Simone Carradori; Marcello Locatelli; Giuliana Vinci; Mattia Rapa; Salvatore Ciano; Anna Maria Giusti; Bruno Botta; Francesca Ghirga; Donatella Capitani; Luisa Mannina

doi:10.3390/molecules25081908

Molecules (Apr 2020)

<i>Cannabis sativa</i> L. Inflorescences from Monoecious Cultivars Grown in Central Italy: An Untargeted Chemical Characterization from Early Flowering to Ripening

Cinzia Ingallina,
Anatoly P. Sobolev,
Simone Circi,
Mattia Spano,
Caterina Fraschetti,
Antonello Filippi,
Antonella Di Sotto,
Silvia Di Giacomo,
Giulia Mazzoccanti,
Francesco Gasparrini,
Deborah Quaglio,
Enio Campiglia,
Simone Carradori,
Marcello Locatelli,
Giuliana Vinci,
Mattia Rapa,
Salvatore Ciano,
Anna Maria Giusti,
Bruno Botta,
Francesca Ghirga,
Donatella Capitani,
Luisa Mannina

Affiliations

Cinzia Ingallina: Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Anatoly P. Sobolev: Institute for Biological Systems, Magnetic Resonance Laboratory “Segre-Capitani”, CNR, Via Salaria Km 29.300, 00015 Monterotondo, Italy
Simone Circi: Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Mattia Spano: Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Caterina Fraschetti: Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Antonello Filippi: Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Antonella Di Sotto: Department of Physiology and Pharmacology “V. Ersparmer”, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Silvia Di Giacomo: Department of Physiology and Pharmacology “V. Ersparmer”, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Giulia Mazzoccanti: Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Francesco Gasparrini: Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Deborah Quaglio: Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Enio Campiglia: Department of Agriculture and Forest Sciences, University of Tuscia, Via San Camillo de Lellis snc, 01100 Viterbo, Italy
Simone Carradori: Department of Pharmacy, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy
Marcello Locatelli: Department of Pharmacy, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy
Giuliana Vinci: Department of Management, Sapienza University of Rome, via del Castro Laurenziano 9, 00161 Rome, Italy
Mattia Rapa: Department of Management, Sapienza University of Rome, via del Castro Laurenziano 9, 00161 Rome, Italy
Salvatore Ciano: Department of Management, Sapienza University of Rome, via del Castro Laurenziano 9, 00161 Rome, Italy
Anna Maria Giusti: Department of Experimental Medicine, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
Bruno Botta: Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Francesca Ghirga: Center for Life Nano Science@Sapienza, Italian Institute of Technology, Viale Regina Elena 291, 00161 Rome, Italy
Donatella Capitani: Institute for Biological Systems, Magnetic Resonance Laboratory “Segre-Capitani”, CNR, Via Salaria Km 29.300, 00015 Monterotondo, Italy
Luisa Mannina: Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy

DOI: https://doi.org/10.3390/molecules25081908
Journal volume & issue: Vol. 25, no. 8
p. 1908

Abstract

Read online

The chemical composition of the inflorescences from four Cannabis sativa L. monoecious cultivars (Ferimon, Uso-31, Felina 32 and Fedora 17), recently introduced in the Lazio Region, was monitored over the season from June to September giving indications on their sensorial, pharmaceutical/nutraceutical proprieties. Both untargeted (NMR) and targeted (GC/MS, UHPLC, HPLC-PDA/FD and spectrophotometry) analyses were carried out to identify and quantify compounds of different classes (sugars, organic acids, amino acids, cannabinoids, terpenoids, phenols, tannins, flavonoids and biogenic amines). All cultivars in each harvesting period showed a THC content below the Italian legal limit, although in general THC content increased over the season. Citric acid, malic acid and glucose showed the highest content in the late flowering period, whereas the content of proline drastically decreased after June in all cultivars. Neophytadiene, nerolidol and chlorogenic acid were quantified only in Felina 32 cultivar, characterized also by a very high content of flavonoids, whereas alloaromadendrene and trans-cinnamic acid were detected only in Uso-31 cultivar. Naringenin and naringin were present only in Fedora 17 and Ferimon cultivars, respectively. Moreover, Ferimon had the highest concentration of biogenic amines, especially in July and August. Cadaverine was present in all cultivars but only in September. These results suggest that the chemical composition of Cannabis sativa L. inflorescences depends on the cultivar and on the harvesting period. Producers can use this information as a guide to obtain inflorescences with peculiar chemical characteristics according to the specific use.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

About the journal

Abstract

Keywords