Revista Colombiana de Biotecnología (Nov 2012)
Potencial probiótico de cepas nativas para uso como aditivos en la alimentación avícola
Abstract
/* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Título en ingles: Probiotic potential of native strains, for as feed additives for poultry Resumen: Se evaluó in vitro el potencial probiótico de cepas nativas aisladas de las heces de pollos asilvestrados (Gallus gallus) pertenecientes a los géneros Lactobacillus sp, Bacillus sp y levaduras tipo saccharomyces sp; se determinó la actividad probiótica mediante pruebas de resistencia al ácido (pH 3, 4, 5, 6, 7), sales de bilis (0,05, 0,1, 0,15, 0.3 %), tolerancia al NaCl (2, 4, 7, 10 %), actividad antagónica (Salmonella sp, E. coli), determinación del tipo de fermentación, crecimiento a temperaturas (28,37,43°C) y capacidad de crecimiento. Las cepas con mayor tolerancia se identificaron a través de pruebas bioquímicas y fermentación de carbohidratos. Como resultado se observó que tres microorganismos: Saccharomyces sp. (3), Bacillus sp. (7) y Lactobacillus sp. (14) poseen propiedades probióticas. Palabras claves: Gallus gallus; Saccharomyces sp.; Bacillus sp.; Lactobacillus sp.; aditivos microbianos. Abstract: Was evaluated in vitro the probiotic potential of native strains isolated from feces of wild chickens (Gallus gallus) belonging to the genera Lactobacillus sp., Bacillus sp. and Saccharomyces ; probiotic activity was determined by testing acid resistance (pH 3, 4, 5.6, 7), bile salts (0,05, 0,1, 0,15, 0,3%), tolerance to NaCl (2, 4, 7,10%), antagonistic activity (Salmonella spp, E. coli), production gas (glucose), growth temperatures (28, 37, 43 ° C) and growth capacity. The most tolerant strains were identified by biochemical tests and carbohydrate fermentation. As a result it was found that three microorganisms: Saccharomyces sp. (3), Bacillus sp. (7) and Lactobacillus sp. (14) have probiotic properties. Key words: Gallus gallus; Saccharomyces sp.; Bacillus sp.; Lactobacillus sp.; microbial additives.
