Laboratory sprayer for dsRNA application: Design and bioassay validation

Abstract

The shortage of commercially available and reliable laboratory spraying equipment for testing different preparations can be a major obstacle to achieve field-comparable results in the laboratory conditions.RNA interference is natural biological process which, when used for plant protection, can be designed method combining sustainability and minimal environmental side effects. Spraying of dsRNA is a field-relevant method that should ensure consistency and repeatability if conducted in laboratory.We built a portable spray device for laboratory use and tested its suitability for dsRNA application. For that, we carried out bioassay on three plant species with different leaf surface textures. DsRNA were detected in all samples 3 days post-treatment indicating its suitability for dsRNA delivery.We built a portable spray device for laboratory use and tested its suitability for dsRNA application. For that, we carried out: • Bioassay on three plant species with different leaf surface textures.DsRNA were detected in all samples 3 days post-treatment indicating its suitability for dsRNA delivery.

Keywords

• SIGS- Spray-Induced Gene Silencing