PCV2 Triggers PK-15 Cell Apoptosis Through the PLC–IP3R–Ca2+ Signaling Pathway

Shuo Wang; Shuo Wang; Chen Li; Panpan Sun; Jianli Shi; Xiaoyan Wu; Xiaoyan Wu; Chang Liu; Zhe Peng; Hong Han; Shaojian Xu; Ying Yang; Ying Yang; Yao Tian; Yao Tian; Jiaxin Li; Jiaxin Li; Hongbin He; Jun Li; Jun Li; Jun Li; Zhao Wang

doi:10.3389/fmicb.2021.674907

Frontiers in Microbiology (Jun 2021)

PCV2 Triggers PK-15 Cell Apoptosis Through the PLC–IP3R–Ca2+ Signaling Pathway

Shuo Wang,
Shuo Wang,
Chen Li,
Panpan Sun,
Jianli Shi,
Xiaoyan Wu,
Xiaoyan Wu,
Chang Liu,
Zhe Peng,
Hong Han,
Shaojian Xu,
Ying Yang,
Ying Yang,
Yao Tian,
Yao Tian,
Jiaxin Li,
Jiaxin Li,
Hongbin He,
Jun Li,
Jun Li,
Jun Li,
Zhao Wang

Affiliations

Shuo Wang: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Shuo Wang: College of Life Sciences, Shandong Normal University, Jinan, China
Chen Li: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Panpan Sun: Qingdao Agricultural University, Qingdao, China
Jianli Shi: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Xiaoyan Wu: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Xiaoyan Wu: College of Life Sciences, Shandong Normal University, Jinan, China
Chang Liu: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Zhe Peng: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Hong Han: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Shaojian Xu: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Ying Yang: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Ying Yang: College of Life Sciences, Shandong Normal University, Jinan, China
Yao Tian: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Yao Tian: Qingdao Agricultural University, Qingdao, China
Jiaxin Li: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Jiaxin Li: Qingdao Agricultural University, Qingdao, China
Hongbin He: College of Life Sciences, Shandong Normal University, Jinan, China
Jun Li: Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, China
Jun Li: College of Life Sciences, Shandong Normal University, Jinan, China
Jun Li: Qingdao Agricultural University, Qingdao, China
Zhao Wang: China Institute of Veterinary Drug Control, Beijing, China

DOI: https://doi.org/10.3389/fmicb.2021.674907
Journal volume & issue: Vol. 12

Abstract

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The endoplasmic reticulum (ER) plays an essential role in Ca2+ concentration balance and protein biosynthesis. During infection, the virus needs to complete its life process with the help of ER. At the same time, ER also produces ER stress (ERS), which induces apoptosis to resist virus infection. Our study explored the Ca2+ concentration, ERS, and the apoptosis mechanism after porcine circovirus 2 (PCV2) infection. We show here that PCV2 infection induces the increased cytoplasmic Ca2+ level and PK-15 cell ER swelling. The colocalization of phospholipase C (PLC) and inositol 1,4,5-trisphosphate receptor (IP3R) in the cytoplasm was observed by laser confocal microscopy. Western blot and quantitative polymerase chain reaction experiments confirmed that PLC and IP3R expression levels increased after PCV2 infection, and Ca2+ concentration in the cytoplasm increased after virus infection. These results suggest that PCV2 infection triggers ERS of PK-15 cells via the PLC–IP3R–Ca2+ signaling pathway to promote the release of intracellular Ca2+ and led to cell apoptosis.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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