De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

Wei He; Liang Zhang; Oscar D. Villarreal; Rongjie Fu; Ella Bedford; Jingzhuang Dou; Anish Y. Patel; Mark T. Bedford; Xiaobing Shi; Taiping Chen; Blaine Bartholomew; Han Xu

doi:10.1038/s41467-019-12489-8

Nature Communications (Oct 2019)

De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

Wei He,
Liang Zhang,
Oscar D. Villarreal,
Rongjie Fu,
Ella Bedford,
Jingzhuang Dou,
Anish Y. Patel,
Mark T. Bedford,
Xiaobing Shi,
Taiping Chen,
Blaine Bartholomew,
Han Xu

Affiliations

Wei He: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center
Liang Zhang: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center
Oscar D. Villarreal: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center
Rongjie Fu: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center
Ella Bedford: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center
Jingzhuang Dou: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center
Anish Y. Patel: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center
Mark T. Bedford: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center
Xiaobing Shi: Center for Epigenetics, Van Andel Research Institute
Taiping Chen: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center
Blaine Bartholomew: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center
Han Xu: Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center

DOI: https://doi.org/10.1038/s41467-019-12489-8
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

Read online

Tiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal