Armaghane Danesh Bimonthly Journal (Jun 2020)
Evaluation of the Effects of Pentoxifylline on Stereological Changes in Frozen Ovarian Tissue of Mice
Abstract
Background & aim: Ovarian cryopreservation is a technique in artificial insemination methods, while the ovary is vulnerable to the cryopreservation process. Pentoxifylline as an antioxidant takes a protective effect by reducing free radicals in culture medium. The aim of the present study was to determine and evaluate the antioxidant effects of pentoxifylline on stereological changes in frozen ovarian tissue of mice after freezing. Methods: The present experimental study was conducted on 25 female ovaries at Shiraz University of Medical Sciences in 2019, which were randomly divided into five groups of five. Group 1 (control): No treatment was performed in this group. Groups 2 and 3 (sham 1,2): In Group 2, after the ovaries were removed, they were placed for half an hour in a CO2 incubator in the saline phosphate buffer + bovine serum albumin (PB1 + BSA) environment, And in Group 3, in addition to being placed in the incubator, 1.8 mM of Pentoxifylline was added to the buffer environment. Groups 4 and 5 (Experimental 1, 2): In Group 4, after the ovaries were removed from the animalchr('39')s body, the ovaries were vitrified for two weeks, after which time they were melted and placed in a CO2 incubator for half an hour in the saline phosphate buffer + bovine serum albumin (PB1 + BSA) environment, and In Group 5, in addition to vitrified, melting, and incubation, the ovaries were exposed to buffers containing 1.8 mM pentoxifylline. After tissue processing, ovarian tissue sections were studied with stereological methods. Data were analyzed using SPSS software and Kruskal-Wallis statistical test. Results: The stereological estimation indicated that the total volume of ovaries and cortex increased in control groups 2, experiment 1 and experiment 2 compared to the control group, but no significant increase was perceived (p<0.05). On the other hand, the volume of medulla in control groups 2, experiment 1 and experiment 2 decreased compared to the control group, but this decrease was not significant (p<0.05). Conclusions: According to the results of the present study, pentoxifylline and cryopreservation increased the volume of ovarian tissue, but despite the antioxidant properties of pentoxifylline, no effect was observed on protecting the ovary from the negative effects of cryopreservation.
