5′ capped and 3′ polyA-tailed sgRNAs enhance the efficiency of CRISPR-Cas9 system

• Wei Mu,
• Yongping Zhang,
• Xutong Xue,
• Lei Liu,
• Xiaofei Wei,
• Haoyi Wang

Affiliations

Wei Mu

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences

Yongping Zhang

Department of Hematology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University

Xutong Xue

Hebei University

Lei Liu

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences

Xiaofei Wei

Beijing Cord Blood Bank

Haoyi Wang

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences