Metal-supported self-assembled bilayer lipid membrane incorporated with peroxidase for the detection of peroxide

Abstract

This work presents a simple, low-cost and ready-to-use peroxide biosensor based on a metal-supported, self-assembled lipid membrane incorporated with horseradish peroxidase. The peroxide sensor showed good analytical and operational characteristics: the detection limit (as S/N = 3) was 0.083 μM and the sensitivity was 1.4071 nA per μM of analyte concentration. The sensor was stable (<5% drift) for 9 h of continuous operation. The validation in real water samples showed that the sensor could reliably detect 0.95 mM of hydrogen peroxide in tap, river and lake water. The reliability of the sensor has been also proven on commercial peroxide formulations, assayed without pretreatment. The prospects and applicability of the proposed biosensor and the underlying technology are also discussed.

Keywords

• Lipid membrane biosensor
• Self-assembly
• Horseradish peroxidase
• Water monitoring
• Hydrogen peroxide