Biochemical parameters in rats with an applied model of sepsis (cecal ligation and puncture) with pure and mixed bacterial culture

Abstract

The goal of this work was to induce clinical sepsis in rats in order to measure the following biochemical parameters (glucoses, triglycerides, cholesterol, total proteins, albumins and creatinine). The experiments were done on 104 male Wistar rats, body weight 190 to 240 g. The rats were divided in four groups. Treated groups consisted of 28 animals while in the control groups there were 20 animals. Animals were observed and sacrificed at 12, 24, 72 and 120 hours after surgery. In this model of sepsis (cecal ligation and puncture - CLP) with pure and mixed bacterial cultures, significant changes were noticed in all biochemical parameters. Significant hypoglycemia, hypercholesterolemia, decreased concentration of urea and increased concentration of creatinine were found in the first half of the experiment in all groups of rats with sepsis (at 12 and 24 hours). Hyperproteinemia and hypotriglyceridemia reached statistically significant values at 24 h in the groups of rats in which sepsis was induced with pure bacteria culture. In the other half of the experiment significant hypoalbuminemia was found in all rats with sepsis (at 71 and 120 h). [Projekat Ministarstva nauke Republike Srbije, br. TR 31071]

Keywords

• biochemical parameters
• Escherichia coli
• rats
• sepsis
• Staphylococcus aureus