iScience (May 2024)

MHC class I-dressing is mediated via phosphatidylserine recognition and is enhanced by polyI:C

  • Arisa Hori,
  • Saori Toyoura,
  • Miyu Fujiwara,
  • Ren Taniguchi,
  • Yasutaka Kano,
  • Tomoyoshi Yamano,
  • Rikinari Hanayama,
  • Masafumi Nakayama

Journal volume & issue
Vol. 27, no. 5
p. 109704

Abstract

Read online

Summary: In addition to cross-presentation, cross-dressing plays an important role in the induction of CD8+ T cell immunity. In the process of cross-dressing, conventional dendritic cells (DCs) acquire major histocompatibility complex class I (MHCI) from other cells and subsequently prime CD8+ T cells via the pre-formed antigen-MHCI complexes without antigen processing. However, the mechanisms underlying the cross-dressing pathway, as well as the relative contributions of cross-presentation and cross-dressing to CD8+ T cell priming are not fully understood. Here, we demonstrate that DCs rapidly acquire MHCI-containing membrane fragments from dead cells via the phosphatidylserine recognition-dependent mechanism for cross-dressing. The MHCI dressing is enhanced by a TLR3 ligand polyinosinic-polycytidylic acid (polyI:C). Further, polyI:C promotes not only cross-presentation but also cross-dressing in vivo. Taken together, these results suggest that cross-dressing as well as cross-presentation is involved in inflammatory diseases associated with cell death and type I IFN production.

Keywords