Nature Communications (Dec 2022)

PEAC-seq adopts Prime Editor to detect CRISPR off-target and DNA translocation

  • Zhenxing Yu,
  • Zhike Lu,
  • Jingjing Li,
  • Yingying Wang,
  • Panfeng Wu,
  • Yini Li,
  • Yangfan Zhou,
  • Bailun Li,
  • Heng Zhang,
  • Yingzheng Liu,
  • Lijia Ma

DOI
https://doi.org/10.1038/s41467-022-35086-8
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 13

Abstract

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It is still a challenge to accurately identify off-target endonuclease edits. Here the authors report PEAC-seq using a Prime Editor to insert a tag to the editing sites and enrich the tagged regions with site-specific primers for sequencing: they show that PEAC-seq could identify DNA translocations.