Nature Communications (Jul 2019)
Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues
- Adam K. Glaser,
- Nicholas P. Reder,
- Ye Chen,
- Chengbo Yin,
- Linpeng Wei,
- Soyoung Kang,
- Lindsey A. Barner,
- Weisi Xie,
- Erin F. McCarty,
- Chenyi Mao,
- Aaron R. Halpern,
- Caleb R. Stoltzfus,
- Jonathan S. Daniels,
- Michael Y. Gerner,
- Philip R. Nicovich,
- Joshua C. Vaughan,
- Lawrence D. True,
- Jonathan T. C. Liu
Affiliations
- Adam K. Glaser
- Department of Mechanical Engineering, University of Washington
- Nicholas P. Reder
- Department of Pathology, University of Washington
- Ye Chen
- Department of Mechanical Engineering, University of Washington
- Chengbo Yin
- Department of Mechanical Engineering, University of Washington
- Linpeng Wei
- Department of Mechanical Engineering, University of Washington
- Soyoung Kang
- Department of Mechanical Engineering, University of Washington
- Lindsey A. Barner
- Department of Mechanical Engineering, University of Washington
- Weisi Xie
- Department of Mechanical Engineering, University of Washington
- Erin F. McCarty
- Department of Pathology, University of Washington
- Chenyi Mao
- Department of Chemistry, University of Washington
- Aaron R. Halpern
- Department of Chemistry, University of Washington
- Caleb R. Stoltzfus
- Department of Immunology, University of Washington
- Jonathan S. Daniels
- Applied Scientific Instrumentation
- Michael Y. Gerner
- Department of Immunology, University of Washington
- Philip R. Nicovich
- Allen Institute for Brain Science
- Joshua C. Vaughan
- Department of Chemistry, University of Washington
- Lawrence D. True
- Department of Pathology, University of Washington
- Jonathan T. C. Liu
- Department of Mechanical Engineering, University of Washington
- DOI
- https://doi.org/10.1038/s41467-019-10534-0
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 8
Abstract
Light-sheet microscopes are increasingly used for imaging cleared tissues, but have imposed constraints on sample geometries and protocols. Here the authors present a multi-immersion open-top light-sheet microscope to overcome these limitations and enable high-throughput imaging of samples processed with various clearing protocols.
