Lith6

Malcolm A. Lyons; Henning Wittenburg; Renhua Li; Kenneth A. Walsh; Monika R. Leonard; Ron Korstanje; Gary A. Churchill; Martin C. Carey; Beverly Paigen

Journal of Lipid Research (Sep 2003)

Lith6

Malcolm A. Lyons,
Henning Wittenburg,
Renhua Li,
Kenneth A. Walsh,
Monika R. Leonard,
Ron Korstanje,
Gary A. Churchill,
Martin C. Carey,
Beverly Paigen

Affiliations

Malcolm A. Lyons: The Jackson Laboratory, Bar Harbor, ME 04609; Department of Medicine, Harvard Medical School, Division of Gastroenterology, Brigham and Women's Hospital, Harvard Digestive Diseases Center, Boston, MA 02115
Henning Wittenburg: The Jackson Laboratory, Bar Harbor, ME 04609; Department of Medicine, Harvard Medical School, Division of Gastroenterology, Brigham and Women's Hospital, Harvard Digestive Diseases Center, Boston, MA 02115
Renhua Li: The Jackson Laboratory, Bar Harbor, ME 04609; Department of Medicine, Harvard Medical School, Division of Gastroenterology, Brigham and Women's Hospital, Harvard Digestive Diseases Center, Boston, MA 02115
Kenneth A. Walsh: The Jackson Laboratory, Bar Harbor, ME 04609; Department of Medicine, Harvard Medical School, Division of Gastroenterology, Brigham and Women's Hospital, Harvard Digestive Diseases Center, Boston, MA 02115
Monika R. Leonard: The Jackson Laboratory, Bar Harbor, ME 04609; Department of Medicine, Harvard Medical School, Division of Gastroenterology, Brigham and Women's Hospital, Harvard Digestive Diseases Center, Boston, MA 02115
Ron Korstanje: The Jackson Laboratory, Bar Harbor, ME 04609; Department of Medicine, Harvard Medical School, Division of Gastroenterology, Brigham and Women's Hospital, Harvard Digestive Diseases Center, Boston, MA 02115
Gary A. Churchill: The Jackson Laboratory, Bar Harbor, ME 04609; Department of Medicine, Harvard Medical School, Division of Gastroenterology, Brigham and Women's Hospital, Harvard Digestive Diseases Center, Boston, MA 02115
Martin C. Carey: The Jackson Laboratory, Bar Harbor, ME 04609; Department of Medicine, Harvard Medical School, Division of Gastroenterology, Brigham and Women's Hospital, Harvard Digestive Diseases Center, Boston, MA 02115
Beverly Paigen: The Jackson Laboratory, Bar Harbor, ME 04609; Department of Medicine, Harvard Medical School, Division of Gastroenterology, Brigham and Women's Hospital, Harvard Digestive Diseases Center, Boston, MA 02115

Journal volume & issue: Vol. 44, no. 9
pp. 1763 – 1771

Abstract

Read online

A complex genetic basis determines the individual predisposition to develop cholesterol gallstones in response to environmental factors. We employed quantitative trait locus/loci (QTL) analyses of an intercross between inbred strains CAST/Ei (susceptible) and DBA/2J (resistant) to determine the subset of gallstone susceptibility (Lith) genes these strains possess. Parental and first filial generation mice of both genders and male intercross offspring were evaluated for gallstone formation after feeding a lithogenic diet. Linkage analysis was performed using a form of multiple interval mapping. One significant QTL colocalized with Lith1 [chromosome (chr) 2, 50 cM], a locus identified previously. Significantly, new QTL were detected and named Lith10 (chr 6, 4 cM), Lith6 (chr 6, 54 cM), and Lith11 (chr 8, 58 cM).Statistical and genetic analyses suggest that Lith6 comprises two QTL in close proximity. Our molecular and genetic data support the candidacy of peroxisome proliferator-activated receptor γ (Pparg) and Slc21a1, encoding Pparg, and the basolateral bile acid transporter SLC21A1 (Slc21a1/Oatp1), respectively, as genes underlying Lith6.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

About the journal

Abstract

Keywords