Nature Communications (Jul 2016)

Genetically encoded protein photocrosslinker with a transferable mass spectrometry-identifiable label

  • Yi Yang,
  • Haiping Song,
  • Dan He,
  • Shuai Zhang,
  • Shizhong Dai,
  • Shixian Lin,
  • Rong Meng,
  • Chu Wang,
  • Peng R. Chen

DOI
https://doi.org/10.1038/ncomms12299
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 10

Abstract

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Mapping protein-protein interaction using crosslinking and mass spectroscopy strategies is hampered by a high rate of false-positive results. Here, the authors develop a genetically encoded photo-affinity probe for accurate identification of protein interaction partners and crosslinking sites.