Antibiofilm efficacy of root-end filling materials against Enterococcus faecalis - An in vitro study

Abstract

Aim of the Study: This study aims to evaluate the antibiofilm activity of root end materials against Enterococcus faecalis. Materials and Methods: Mineral trioxide aggregate (MTA), MTA plus and Biodentine were conjugated with chitosan gel and tested against the 3-day biofilm of E. faecalis. The incubated plates were stained using crystal violet stain and the optical density of adherent stained biofilm was read at 590 nm using ELISA auto reader. Results: There was a mean clinical reduction in the biofilms of the conjugates as compared to their individual counter parts. There was a statistically significant difference seen between the groups (MTA Plus – Chitosan Conjugate) and (MTA – Chitosan Conjugate) with P = 0.0495. Conclusion: The conjugates did perform better in inhibiting the biofilm activity of E. faecalis. Although all conjugates formed were not statistically significant.

Keywords

• biofilm
• chitosan conjugate
• root-end materials