Frontiers in Cardiovascular Medicine (Sep 2022)
Bmal1 knockdown in the left stellate ganglion inhibits neural activity and prevents ventricular arrhythmias after myocardial ischemia
- Zhongyang Yu,
- Zhongyang Yu,
- Zhongyang Yu,
- Zhongyang Yu,
- Zhongyang Yu,
- Zhongyang Yu,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Liying Jiao,
- Liying Jiao,
- Liying Jiao,
- Liying Jiao,
- Liying Jiao,
- Liying Jiao,
- Song Zhang,
- Song Zhang,
- Song Zhang,
- Song Zhang,
- Song Zhang,
- Song Zhang,
- Liqing Nie,
- Liqing Nie,
- Liqing Nie,
- Liqing Nie,
- Liqing Nie,
- Liqing Nie,
- Yueyi Wang,
- Yueyi Wang,
- Yueyi Wang,
- Yueyi Wang,
- Yueyi Wang,
- Yueyi Wang,
- Liping Zhou,
- Liping Zhou,
- Liping Zhou,
- Liping Zhou,
- Liping Zhou,
- Liping Zhou,
- Yuhong Wang,
- Yuhong Wang,
- Yuhong Wang,
- Yuhong Wang,
- Yuhong Wang,
- Yuhong Wang,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Zhihao Liu,
- Zihan Liu,
- Zihan Liu,
- Zihan Liu,
- Zihan Liu,
- Zihan Liu,
- Zihan Liu,
- Xiao Xu,
- Xiao Xu,
- Xiao Xu,
- Xiao Xu,
- Xiao Xu,
- Xiao Xu,
- Zeyan Li,
- Zeyan Li,
- Zeyan Li,
- Zeyan Li,
- Zeyan Li,
- Zeyan Li,
- Yuyang Zhou,
- Yuyang Zhou,
- Yuyang Zhou,
- Yuyang Zhou,
- Yuyang Zhou,
- Yuyang Zhou,
- Huixin Zhou,
- Huixin Zhou,
- Huixin Zhou,
- Huixin Zhou,
- Huixin Zhou,
- Huixin Zhou,
- Rui Li,
- Rui Li,
- Rui Li,
- Rui Li,
- Rui Li,
- Rui Li,
- Chen Peng,
- Chen Peng,
- Chen Peng,
- Chen Peng,
- Chen Peng,
- Chen Peng,
- Lilei Yu,
- Lilei Yu,
- Lilei Yu,
- Lilei Yu,
- Lilei Yu,
- Lilei Yu,
- Hong Jiang,
- Hong Jiang,
- Hong Jiang,
- Hong Jiang,
- Hong Jiang,
- Hong Jiang
Affiliations
- Zhongyang Yu
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Zhongyang Yu
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Zhongyang Yu
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Zhongyang Yu
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Zhongyang Yu
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Zhongyang Yu
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Zhihao Liu
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Zhihao Liu
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Zhihao Liu
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Zhihao Liu
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Zhihao Liu
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Zhihao Liu
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Liying Jiao
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Liying Jiao
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Liying Jiao
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Liying Jiao
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Liying Jiao
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Liying Jiao
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Song Zhang
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Song Zhang
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Song Zhang
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Song Zhang
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Song Zhang
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Song Zhang
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Liqing Nie
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Liqing Nie
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Liqing Nie
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Liqing Nie
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Liqing Nie
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Liqing Nie
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Yueyi Wang
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Yueyi Wang
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Yueyi Wang
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Yueyi Wang
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Yueyi Wang
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Yueyi Wang
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Liping Zhou
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Liping Zhou
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Liping Zhou
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Liping Zhou
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Liping Zhou
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Liping Zhou
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Yuhong Wang
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Yuhong Wang
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Yuhong Wang
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Yuhong Wang
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Yuhong Wang
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Yuhong Wang
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Zhihao Liu
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Zhihao Liu
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Zhihao Liu
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Zhihao Liu
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Zhihao Liu
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Zhihao Liu
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Zihan Liu
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Zihan Liu
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Zihan Liu
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Zihan Liu
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Zihan Liu
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Zihan Liu
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Xiao Xu
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Xiao Xu
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Xiao Xu
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Xiao Xu
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Xiao Xu
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Xiao Xu
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Zeyan Li
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Zeyan Li
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Zeyan Li
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Zeyan Li
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Zeyan Li
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Zeyan Li
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Yuyang Zhou
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Yuyang Zhou
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Yuyang Zhou
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Yuyang Zhou
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Yuyang Zhou
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Yuyang Zhou
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Huixin Zhou
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Huixin Zhou
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Huixin Zhou
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Huixin Zhou
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Huixin Zhou
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Huixin Zhou
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Rui Li
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Rui Li
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Rui Li
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Rui Li
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Rui Li
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Rui Li
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Chen Peng
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Chen Peng
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Chen Peng
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Chen Peng
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Chen Peng
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Chen Peng
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Lilei Yu
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Lilei Yu
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Lilei Yu
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Lilei Yu
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Lilei Yu
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Lilei Yu
- Hubei Key Laboratory of Cardiology, Wuhan, China
- Hong Jiang
- Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China
- Hong Jiang
- Hubei Key Laboratory of Autonomic Nervous System Modulation, Wuhan, China
- Hong Jiang
- Cardiac Autonomic Nervous System Research Center of Wuhan University, Wuhan, China
- Hong Jiang
- Taikang Center for Life and Medical Sciences, Wuhan University, Wuhan, China
- Hong Jiang
- Cardiovascular Research Institute, Wuhan University, Wuhan, China
- Hong Jiang
- Hubei Key Laboratory of Cardiology, Wuhan, China
- DOI
- https://doi.org/10.3389/fcvm.2022.937608
- Journal volume & issue
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Vol. 9
Abstract
ObjectivesThe neural activity of the left stellate ganglion (LSG) is closely related to the occurrence of ventricular arrhythmias (VAs). Bmal1 modulates genes associated with neural activity in the central nervous system. However, few studies indicated the role of Bmal1 in the LSG and the subsequent effect on the heart. Therefore, we aimed to investigate the influence of Bmal1 knockdown in the LSG on its neural activity and cardiac electrophysiology and to explore the mechanisms.Materials and methodsWe used adeno-associated virus (AAV) to knock down Bmal1 in the LSG. Male beagles were randomized into the Bmal1 knockdown group and the control group. After 4 weeks of injection, the LSG function, neural activity, left ventricular effective refractory period (ERP), and action potential duration (APD) were measured. Electrocardiography for 1 h was recorded for VAs analysis after myocardial ischemia. Nerve growth factor (NGF) and c-fos in the LSG were quantified by immunofluorescence. Transcriptomic analysis was performed to assess the gene expression in the LSG.ResultsBmal1 was sufficiently knocked down by AAV. Compared with the control group, heart rate variability (HRV) in the knockdown group was altered. Bmal1 knockdown inhibited neural activity and function of LSG. It also prolonged ERP as well as APD90. Ischemia-induced VAs were significantly reduced. Nerve growth factor (NGF) and c-fos in the LSG were reduced. Bmal1 knockdown led to the expression changes of genes associated with neural activity in the LSG.ConclusionBmal1 knockdown in the LSG suppresses neural activity and prevents ventricular arrhythmias after myocardial ischemia.
Keywords